Ultrastructural analysis of central serotoninergic neurons immunolabeled by silver-gold-intensified diaminobenzidine chromogen. Completion of immunocytochemistry with X-ray microanalysis
Serotonin immunoreactive structures of the rat central nervous system (CNS) were detected by the recently developed silver-intensified peroxidase-antiperoxidase complex (SI-PAP) method at both the light and electron microscopic levels. The silver postintensification of the diaminobenzidine (DAB) chromogen increased the sensitivity of the original PAP method, resulting in a very Golgi-like appearance of serotonin-immunopositive neuronal elements. The metallic silver and gold deposited onto DAB-labeled organelles, filling out the whole immunoreactive neuron, assures the easy tracing of thin neuronal processes far from the cell body. At the ultrastructural level, metallic grains were seen over immunolabeled structures only, proving the specificity of the silver method. In neurons of the dorsal raphe nucleus, free ribosomes, endoplasmic reticulum, and granules (80-100 nm in diameter) were labeled. Immunoreactive, e.g., serotoninergic, dendrites were seen to receive afferent terminals. The increased electron density of the intensified immunolabel facilitates the ultrastructural recognition of even weakly labeled profiles, while its metallic components (Ag and Au) provide a base for X-ray analysis of the immunolabeled biological specimen.
- OSTI ID:
- 6528490
- Journal Information:
- J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 6; ISSN JHCYA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Radioimmunocytochemistry using a tritiated goat anti-rabbit second antibody
Distribution of 125I-galanin binding sites, immunoreactive galanin, and its coexistence with 5-hydroxytryptamine in the cat spinal cord: Biochemical, histochemical, and experimental studies at the light and electron microscopic level
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BENZIDINE
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
DRUGS
ELECTRON MICROPROBE ANALYSIS
ELEMENTS
ENZYMES
GOLD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTOLOGICAL TECHNIQUES
HYDROXY COMPOUNDS
IMMUNE REACTIONS
INDOLES
MAMMALS
METALS
MICROANALYSIS
MOLECULAR STRUCTURE
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PORPHYRINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SEROTONIN
SILVER
SOMATIC CELLS
SYMPATHOMIMETICS
TRANSITION ELEMENTS
TRYPTAMINES
VERTEBRATES