Distribution of /sup 203/Hg and /sup 75/Se in the egg and egg white proteins
Journal Article
·
· Fed Proc Fed Am Soc Exp Biol; (United States)
OSTI ID:6304452
Laying hens fed a practical diet with no added mercury (Hg) or selenium (Se) were given /sup 203/Hg and /sup 75/Se by oral capsule. During the subsequent 20 days, approximately 50% and 9% of the /sup 203/Hg dose was found in whites and yolks, respectively, of egg produced. Only 35% of the /sup 75/Se dose was recovered in the eggs, the majority occurring in the egg yolk. Incorporation of 20 ppm Hg in the diet produced a two-fold increase in the /sup 75/Se content of the egg whites and a 30% increase in /sup 203/Hg of the egg yolk. Addition of 8 ppm Se to the diet decreased the /sup 75/Se content of whole egg by about 60% and slightly increased the /sup 203/Hg content of the whole egg. Fractionation of the egg white proteins and measurement of the /sup 203/Hg and /sup 75/Se distribution showed that approximately 90% of the /sup 203/Hg recovered was in the ovalbumin fraction while /sup 75/Se occurred mainly in the conalbumin fraction, whether the isotopes were given individually or in combination. Addition of 8 ppm Se and/or 20 ppm Hg to the diet produced no change in the /sup 203/Hg distribution. An apparent shift of the /sup 75/Se binding from the conalbumin to the globulin fraction was observed as a result of addition of Hg and/or Se to the diets.
- Research Organization:
- Iowa State Univ., Ames
- OSTI ID:
- 6304452
- Journal Information:
- Fed Proc Fed Am Soc Exp Biol; (United States), Journal Name: Fed Proc Fed Am Soc Exp Biol; (United States) Vol. 37:3
- Country of Publication:
- United States
- Language:
- English
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