Measurement of mutagenesis in mammalian cells. [X radiation, caffeine, MNNG]
A method using mammalian cells in vitro for detection and quantitation of mutagenic actions that appears to be useful for screening for carcinogenesis and genetic damage by environmental agents is presented. The method involves use of stable human-Chinese hamster ovary hybrid cells that have retained a single human chromosome not necessary for cell reproduction. Forward mutations are detected in genes necessary for production of specific human cell surface antigens. Such mutants form colonies in the presence of specific antisera and complement that destroy the ummutagenized cells. Use of the method is illustrated for the action of x-irradiation, N-methyl-N'-nitro-N-nitrosoguanidine, and caffeine. The method appears to be unique in that it permits assessment of lesions that cause loss of all or most of the chromosome as well as various localized gene mutations. The former action is particularly important because of the major involvement of chromosomal lesions in an extremely important class of human genetic disease.
- Research Organization:
- Univ. of Colorado, Denver
- OSTI ID:
- 6294802
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 76:3; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells
Mutant quantity and quality in mammalian cells (A{sub L}) exposed to cesium-137 gamma radiation: Effect of caffeine
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CAFFEINE
CHROMOSOMAL ABERRATIONS
DATA
DATA FORMS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GRAPHS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAMMALS
MEASURING METHODS
MUTAGENESIS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SURVIVAL CURVES
VERTEBRATES
X RADIATION
XANTHINES