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Effects of hydroxyl radical scavengers KCN and CO on ultraviolet light-induced activation of crude soluble guanylate cyclase

Journal Article · · J. Cyclic Nucleotide Protein Phosphor. Res.; (United States)
OSTI ID:6293616
; ;
The crude soluble guanylate cyclase (GC) from bovine mesenteric artery was stimulated by ultraviolet (UV) light (366 nm). Addition of free radical scavengers, dimethylsulfoxide or superoxide dismutase and/or catalase to the GC assay did not abolish the stimulatory effect of UV light. On the contrary, the UV light-induced activation was enhanced in the presence of these scavengers. KCN (1 mM) did not affect the UV light-induced activation, while 0.1 mM of CO potentiated the activation. These results may indicate that UV light is operating through a direct interaction with the ferrous form of the GC-heme.
Research Organization:
Linkoeping Univ., Sweden
OSTI ID:
6293616
Journal Information:
J. Cyclic Nucleotide Protein Phosphor. Res.; (United States), Journal Name: J. Cyclic Nucleotide Protein Phosphor. Res.; (United States) Vol. 4; ISSN JCNRE
Country of Publication:
United States
Language:
English

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560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
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ORGANIC NITROGEN COMPOUNDS
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OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
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RADIATION EFFECTS
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