Persistence of sister chromatid exchanges and in vitro morphological transformation of Syrian hamster fetal cells by chemical and physical carcinogens

Journal Article · Thu Oct 31 23:00:00 EST 1985 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/6.11.1627· OSTI ID:6293441

Popescu, N C; Amsbaugh, S C; DiPaolo, J A

The induction of neoplastic cell transformation is closely associated with DNA alterations which occur shortly after carcinogen exposure. Sister chromatid exchange (SCE) formation is a sensitive indicator of carcinogen-DNA interaction and correlates with the induction of morphological cell transformation. The persistence of lesions generating SCE produced by chemical and physical carcinogens and its relevance to the induction of morphologic transformation was evaluated in coordinated experiments with cultured Syrian hamster fetal cells (HFC). Exponentially growing HFC were exposed for 1 h to benzo(a)pyrene (BP), methyl-methanesulfonate (MMS), cis-platinum (II) diaminedichloride (cis Pt II), N-methyl-N'-nitrosourea (MNU), mitomycin C (MMC), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), N-acetoxy-2-fluorenyl-acetamide (AcAAF) or u.v. light irradiated. SCE analysis demonstrates that for a period of 48 h after carcinogen exposure, during which time the cells undergo at least four replicative cycles, DNA damage generating SCE induced by all chemical carcinogens either persisted or was partially removed, whereas u.v.-induced lesions were completely removed. An elevated SCE frequency persisted after two additional cell cycles after treatment with BP, AcAAF or MMC without increased cell lethality as compared to other carcinogens whose lesions were completely eliminated during the same period.

• Research Organization: National Cancer Institute, Bethesda, MD
• OSTI ID: 6293441
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 11; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL TRANSFORMATIONS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HAMSTERS
HYDROCARBONS
MAMMALS
METHYL METHANESULFONATE
MITOMYCIN
MUTAGENS
MUTATIONS
NITROSO COMPOUNDS
NITROSOUREAS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLATINUM COMPOUNDS
RADIATIONS
RADIOINDUCTION
RODENTS
SISTER CHROMATID EXCHANGES
SULFONIC ACID ESTERS
TRANSITION ELEMENT COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES