Method for locating and purifying DNA containing single base mismatches
A method is described for detecting guanine and thymine bases which are unpaired according to the Watson-Crick base pairing scheme in a double stranded polynucleotide molecule, each unpaired guanine or thymine base being immediately preceded by at least one base which is paired, and immediately followed by at least one base which is paired, the preceding and following paired bases being on the same polynucleotide sequence as the unpaired guanine or thymine base comprising: (a) reacting the double stranded polynucleotide molecule with a reagent capable of altering the electrophoretic mobility of a double stranded polynucleotide molecule by derivatizing unpaired guanine and thymine bases in the double stranded polynucleotide molecule, wherein the double stranded polynucleotide molecule is not a covalently closed circular DNA; (b) observing the electrophoretic mobility of the double stranded polynucleotide molecule which has been reacted with the reagent; and (c) determining the presence or absence of an alteration in the electrophoretic mobility; whereby the presence or absence of unpaired guanine and thymine bases in the double stranded polynucleotide molecule is detected.
- Assignee:
- Lifecodes Corp., Valhalla, NY
- Patent Number(s):
- US 4794075
- OSTI ID:
- 6292591
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
AROMATICS
AZAARENES
AZINES
DETECTION
DNA
DNA MISMATCH
DNA SEQUENCING
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MOLECULAR BIOLOGY
MOLECULES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURIFICATION
PURINES
PYRIMIDINES
STRUCTURAL CHEMICAL ANALYSIS
THYMINE
URACILS