DNA purification by triplex-affinity capture and affinity capture electrophoresis

Cantor, Charles R; Ito, Takashi; Smith, Cassandra L

DNA purification by triplex-affinity capture and affinity capture electrophoresis

Patent · Sun Dec 31 23:00:00 EST 1995

OSTI ID:870243

Cantor, Charles R ^[1]; Ito, Takashi ^[2]; Smith, Cassandra L ^[1]

Boston, MA
Chiba, JP

The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA

DOE Contract Number:: AC03-76SF00098

Assignee:: Regents of University of California (Oakland, CA)

Patent Number(s):: US 5482836

OSTI ID:: 870243

Country of Publication:: United States

Language:: English

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/435/204/999/
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DNA purification by triplex-affinity capture and affinity capture electrophoresis

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