Importance of bound divalent cations to the tyrosine deprotonation during the photocycle of bacteriorhodopsin
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The transient absorption changes occurring at 297 nm during the photocycles of the deionized and acidified bacteriorhodopsins (blue membranes) were studied. As opposed to what happens during the photocycle of the purple membrane, for the blue membranes only the fast absorption increase corresponding to trans-cis isomerization of the retinal chromophore is present; the slow rise attributed to the tyrosine deprotonation is not observed. The addition of different salts to the deionized membrane restores the original color and causes a tyrosine deprotonation during the photocycle. This suggests that the presence of cations is required for the deprotonation of tyrosine as it is for the deprotonation of the retinylidene Schiff base. These results are discussed in terms of the recently proposed cation model for the observed deprotonation processes in the photocycle of bacteriorhodopsin.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 6292331
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:11; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BACTERIA
BIOPHYSICS
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
ELECTROMAGNETIC RADIATION
FAR ULTRAVIOLET RADIATION
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
IONS
ISOMERIZATION
MAGNESIUM CHLORIDES
MAGNESIUM COMPOUNDS
MEMBRANES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PIGMENTS
PROTEINS
RADIATIONS
RHODOPSIN
SODIUM CHLORIDES
SODIUM COMPOUNDS
SPECTRA
TYROSINE
ULTRAVIOLET RADIATION
VITAMIN A
VITAMINS
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BACTERIA
BIOPHYSICS
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
ELECTROMAGNETIC RADIATION
FAR ULTRAVIOLET RADIATION
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
IONS
ISOMERIZATION
MAGNESIUM CHLORIDES
MAGNESIUM COMPOUNDS
MEMBRANES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PIGMENTS
PROTEINS
RADIATIONS
RHODOPSIN
SODIUM CHLORIDES
SODIUM COMPOUNDS
SPECTRA
TYROSINE
ULTRAVIOLET RADIATION
VITAMIN A
VITAMINS