Time-resolved laser studies on the proton pump mechanism of bacteriorhodopsin. Progress report, December 1, 1985-November 30, 1986
Technical Report
·
OSTI ID:5463710
The photocycle of bacteriorhodopsin (bR) and its perturbed forms were investigated by a time resolved resonance Raman study. These experiments were performed in the C = C stretching and in the fingerprint spectral regions for the acid blue, acid purple and deionized forms of bR. The main observations were that isomerization of the retinal, from all-trans to 13-cis, occurs in all bR, formation of the early K/sub 610/ and L/sub 550/ analogues also occur in all bR, and deprotonation of the protonated Schiff base (PSB) only occurs in native bR, but is inhibited in all perturbed bR species. The deprotonation kinetics of tyrosine and the protonated Schiff base during the bacteriorhodopsin photocycle were studied under different perturbations by transient absorption spectroscopy.
- Research Organization:
- California Univ., Los Angeles (USA). Dept. of Chemistry and Biochemistry
- DOE Contract Number:
- AS03-78ER70278
- OSTI ID:
- 5463710
- Report Number(s):
- UCLA-34P278-54; ON: DE86014994
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CONFORMATIONAL CHANGES
HYDROXY ACIDS
MEMBRANE TRANSPORT
MOLECULAR BIOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
RAMAN SPECTRA
RHODOPSIN
SPECTRA
TYROSINE
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CONFORMATIONAL CHANGES
HYDROXY ACIDS
MEMBRANE TRANSPORT
MOLECULAR BIOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
RAMAN SPECTRA
RHODOPSIN
SPECTRA
TYROSINE