Temperature and nucleotide dependence of calcium release by myo-inositol 1,4,5-trisphosphate in cultured vascular smooth muscle cells

Journal Article · Mon Nov 25 00:00:00 EST 1985 · J. Biol. Chem.; (United States)

OSTI ID:6292299

Smith, J B; Smith, L; Higgins, B L

Inositol 1,4,5-trisphosphate (IP3) rapidly increased UVCaS efflux from a nonmitochondrial organelle in cultured vascular smooth muscle cells that were permeabilized with saponin. A nucleotide, preferably ATP, was essential for IP3-evoked UVCaS release. Two nonhydrolyzable ATP analogues satisfied the nucleotide requirement for IP3-evoked UVCaS release. IP3 strongly stimulated UVCaS efflux at low temperatures (1 to 15 degrees C). Decreasing the temperature from 37 to 4 degrees C inhibited the rate of IP3-stimulated efflux by only about 33%. The failure of such low temperatures to strongly inhibit IP3-induced UVCaS efflux suggests that IP3 activated a CaS channel, rather than a carrier, by a ligand-binding, rather than a metabolic, reaction.

• Research Organization: Univ. of Alabama, Birmingham
• OSTI ID: 6292299
• Journal Information: J. Biol. Chem.; (United States), Vol. 27
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
MEMBRANE TRANSPORT
ATP
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CATIONS
CELL CULTURES
INOSITOL
MICE
MUSCLES
NUCLEOTIDES
PERMEABILITY
SAPONINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CARBOHYDRATES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GLYCOSIDES
INOSITOLS
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METALS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques