Nuclear pore complex: novel glycoprotein constituents

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6292253

Davis, L I

The author describes the production of anti-nuclear monoclonal antibodies, and their use in identifying some of the constituents of the nuclear pore complex. Studies are described using an antibody that recognizes predominantly a 62 kD protein (p62) of rat liver nuclei. This protein remains associated with the nuclear pore complex-lamina fraction that results from treatment of nuclei with DNase, RNase and nonionic detergent. Immunofluorescence microscopy revealed a strikingly punctate pattern of nuclear rim staining. Using immunogold electron microscopy, p62 was specifically localized to the pore complex. Pulse chase analysis of labelled tissue culture cells showed that p62 is synthesized as a soluble cytoplasmic precursor. Additionally, p62 is posttranslationally modified by the addition of GlcNAc residues. These proteins have been shown to contain O-linked monosaccharidic GlcNAc residues. Pulse chase labelling of tissue culture cells with /sup 35/S-methionine indicates that most of the sugar is added within 5 min of synthesis, when p62 is soluble and cytosolic. Tunicamycin does not prevent glycosylation and the M/sub r/ of p62 is not affected by endoglycosidase H. Thus, the addition of GlcNAc appears to be carried out by a cytoplasmically disposed transferase and is clearly distinct from the N- and O-linked glycosylation pathways in the endoplasmic reticulum and Golgi complex.

• Research Organization: Rockefeller Univ., New York (USA)
• OSTI ID: 6292253
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL NUCLEI
CHEMICAL COMPOSITION
GLUCOPROTEINS
BIOSYNTHESIS
BIOLOGICAL PATHWAYS
CYTOPLASM
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
LIVER
METHIONINE
MONOCLONAL ANTIBODIES
RATS
SULFUR 35
TRACER TECHNIQUES
TRANSFERASES
AMINO ACIDS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GLANDS
GLYCOPROTEINS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MICROSCOPY
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques