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Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Using a monoclonal antibody (mAb 414), the authors previously identified a protein of 62 kDa (p62) that was localized to the nuclear pore complex by immunoelectron microscopy. They also showed that p62 binds specifically to wheat germ agglutinin. Therefore, they proposed that this nuclear pore complex protein might be a member of a recently characterized family of glycoproteins that are labeled by in vitro galactosylation of rat liver nuclei and contain O-linked monosaccharidic GlcNAc residues. In support of this, they now show that incubation with N-acetylglucosaminidase reduces the molecular mass of p62 by approx. = 3 kDa because of the removal of terminal GlcNAc residues. Moreover, p62 can be galactosylated in vitro by using UDP-(/sup 3/H)galactose and galactosyltransferase. They also show that most of the GlcNAc residues are added within 5 min of synthesis, when p62 is soluble and cytosolic. Thus, the addition of GlcNAc is carried out in the cytoplasm and is clearly distinct from the N- and O-linked glycosylation pathways of the endoplasmic reticulum and Golgi complex. Using another mAb with a broad specificity for nuclear GlcNAc-containing proteins, they show by immunofluorescence and protein blotting of subnuclear fractions that some of these proteins are in the interior of the nucleus, and others are most likely located in the pore complex.

Research Organization:
Rockefeller Univ., New York, NY (USA)
OSTI ID:
6771322
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:21; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
FLUORESCENCE
GALACTOSE
GLUCOPROTEINS
GLYCOPROTEINS
GLYCOSYL TRANSFERASES
HEXOSES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
METHIONINE
MONOCLONAL ANTIBODIES
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
TRANSFERASES
TRITIUM COMPOUNDS