Identification and characterization of an ATP. Mg-dependent protein phosphatase from pig brain
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6292038
Substantial amounts of ATP.Mg-dependent phosphorylase phosphatase (Fc. M) and its activator (kinase FA) were identified and extensively purified from pig brain, in spite of the fact that glycogen metabolism in the brain is of little importance. The brain Fc.M was completely inactive and could only be activated by ATP.Mg and FA, isolated either from rabbit muscle or pig brain. Kinetical analysis of the dephosphorylation of endogenous brain protein indicates that Fc.M could dephosphorylate TSP-labeled myelin basic protein (MBP) and (TSP)phosphorylase alpha at a comparable rate and moreover, this associated MBP phosphatase activity was also strictly kinase FA/ATP.Mg-dependent, demonstrating that MBP is a potential substrate for Fc.M in the brain. By manipulating MBP and inhibitor-2 as specific potent phosphorylase phosphatase inhibitors, we further demonstrate that 1) Fc.M contains two distinct catalytic sites to dephosphorylate different substrates, and 2) brain MBP may be a physiological trigger involved in the regulation of protein phosphatase substrate specificity in mammalian nervous tissues.
- Research Organization:
- National Tsing Hua Univ., Taiwan, China
- OSTI ID:
- 6292038
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 25; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERASES
GLYCOGEN
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
MAMMALS
MUSCLES
MYELIN
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POLYSACCHARIDES
PROTEINS
PURIFICATION
RABBITS
RADIOISOTOPES
SACCHARIDES
SUBSTRATES
SWINE
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERASES
GLYCOGEN
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
MAMMALS
MUSCLES
MYELIN
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POLYSACCHARIDES
PROTEINS
PURIFICATION
RABBITS
RADIOISOTOPES
SACCHARIDES
SUBSTRATES
SWINE
TRACER TECHNIQUES
VERTEBRATES