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Selective dephosphorylation of histone H1 by nuclear phosphatases

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6285738
;
The aim of this study was to characterize the sites of H1 phosphorylated by the cAMP-dependent protein kinase (kinase A) and the CaS phospholipid-dependent protein kinase (kinase C) and to study their dephosphorylation by nuclear protein phosphatases. H1 was phosphorylated on a ser residue to approx. 1 mole (TSP)/mole H1 with either kinase A or C. The sites of phosphorylation were differentiated by digestion of the H1 by thrombin or N-bromosuccinimide. Phosphopeptide maps on reversed phase HPLC and gel filtration HPLC clearly showed that the kinase C phosphorylated a different site than the well characterized kinase A site. H1, phosphorylated by kinase C or kinase A, was used as a substrate for the nuclear phosphatases. The nuclear phosphatases were purified from salt extracted rat liver chromatin and separated into 2 forms based on heat-stable inhibitor sensitivity and polycation stimulation. Polycation-stimulated phosphatase rapidly dephosphorylated the kinase C site and slowly dephosphorylated the kinase A site. The inhibitor-sensitive enzyme showed little activity toward either site under standard assay conditions.
Research Organization:
Medical College of Ohio, Toledo
OSTI ID:
6285738
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERASES
FRACTIONATION
HISTONES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES