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Title: Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus

Abstract

Effects of vasoactive intestinal polypeptide (VIP) on the release of acetylcholine (ACh) from longitudinal muscle strips with myenteric plexus (LM) preparations were examined in the guinea pig small intestine. VIP (10 to 10 W M) induced a concentration-dependent contraction of LM preparation. The VIP-induced contractions seem to be related to three components, the scopolamine-sensitive, the scopolamine-insensitive, the tetrodotoxin-sensitive, and the tetrodotoxin-insensitive contractions. VIP (10 to 10 W M) induced a concentration-dependent increase in the release of (TH)ACh from LM preparations preloaded with (TH)choline. The VIP-evoked (TH)ACh release was inhibited by removal of CaS from the perfusion medium and by treatment with tetrodotoxin but not by scopolamine and hexamethonium. The spontaneous and VIP-evoked (TH)ACh release was not affected by phentolamine, propranolol, methysergide, diphenhydramine, cimetidine, bicuculline, or (D-ProS, D-Trp/sup 7,9/)substance P. The result demonstrates that VIP induces contractions of longitudinal smooth muscle directly and indirectly by the stimulation of both cholinergic neurons and noncholinergic excitatory neurons.

Authors:
; ; ;
Publication Date:
Research Org.:
Kobe Univ. School of Medicine, Japan
OSTI Identifier:
6291772
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 251:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ACETYLCHOLINE; SECRETION; POLYPEPTIDES; BIOLOGICAL EFFECTS; TRITIUM COMPOUNDS; CARDIOVASCULAR AGENTS; CHOLINE; DOSE-RESPONSE RELATIONSHIPS; GUINEA PIGS; MUSCLES; SMALL INTESTINE; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; DIGESTIVE SYSTEM; DRUGS; ESTERS; GASTROINTESTINAL TRACT; HYDROXY COMPOUNDS; INTESTINES; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANS; PARASYMPATHOMIMETICS; PEPTIDES; PROTEINS; QUATERNARY COMPOUNDS; RODENTS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Kusunoki, M., Tsai, L.H., Taniyama, K., and Tanaka, C. Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus. United States: N. p., 1986. Web.
Kusunoki, M., Tsai, L.H., Taniyama, K., & Tanaka, C. Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus. United States.
Kusunoki, M., Tsai, L.H., Taniyama, K., and Tanaka, C. Tue . "Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus". United States.
@article{osti_6291772,
title = {Vasoactive intestinal polypeptide provokes acetylcholine release from the myenteric plexus},
author = {Kusunoki, M. and Tsai, L.H. and Taniyama, K. and Tanaka, C.},
abstractNote = {Effects of vasoactive intestinal polypeptide (VIP) on the release of acetylcholine (ACh) from longitudinal muscle strips with myenteric plexus (LM) preparations were examined in the guinea pig small intestine. VIP (10 to 10 W M) induced a concentration-dependent contraction of LM preparation. The VIP-induced contractions seem to be related to three components, the scopolamine-sensitive, the scopolamine-insensitive, the tetrodotoxin-sensitive, and the tetrodotoxin-insensitive contractions. VIP (10 to 10 W M) induced a concentration-dependent increase in the release of (TH)ACh from LM preparations preloaded with (TH)choline. The VIP-evoked (TH)ACh release was inhibited by removal of CaS from the perfusion medium and by treatment with tetrodotoxin but not by scopolamine and hexamethonium. The spontaneous and VIP-evoked (TH)ACh release was not affected by phentolamine, propranolol, methysergide, diphenhydramine, cimetidine, bicuculline, or (D-ProS, D-Trp/sup 7,9/)substance P. The result demonstrates that VIP induces contractions of longitudinal smooth muscle directly and indirectly by the stimulation of both cholinergic neurons and noncholinergic excitatory neurons.},
doi = {},
journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 251:1,
place = {United States},
year = {1986},
month = {7}
}