Impaired acetylcholine release from the myenteric plexus of Trichinella-infected rats

Journal Article · Thu Nov 30 23:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:7105256

Collins, S M; Blennerhassett, P A; Blennerhassett, M G; Vermillion, D L ^[1]

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1. McMaster Univ., Hamilton, Ontario (Canada)

We examined the release of acetylcholine (ACh) from jejunal longitudinal muscle-myenteric plexus preparations in noninfected control rats and in rats infected 6, 23, or 40 days previously with Trichinella spiralis. ACh release was assessed by preincubating the tissue with ({sup 3}H)choline and measuring the evoked release of tritium. The uptake of {sup 3}H was significantly less in tissue from T. spiralis-infected rats compared with control. In tissues from either infected or control animals, electrical field stimulation (30 V, 0.5 ms, 10 Hz for 1 min), or veratridine (6-30 microM) induced {sup 3}H release that was tetrodotoxin sensitive. Depolarization by KCl (25-75 mM) also caused {sup 3}H release, but this was only partially reduced by tetrodotoxin. Radiochromatographic analysis indicated evoked release of {sup 3}H to be almost entirely ({sup 3}H)ACh. In rats infected 6 days previously with T. spiralis, ({sup 3}H)ACh release induced by KCl, veratridine, and field stimulation were decreased at least 80%. The suppression of ({sup 3}H)ACh release induced by veratridine or KCl was fully reversible after 40 days postinfection, but field-stimulated responses remained approximately 50% of control values. These results indicate that T. spiralis infection in the rat is accompanied by a reversible suppression of ACh release from the longitudinal muscle-myenteric plexus of the jejunum.

• OSTI ID: 7105256
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
ASCHELMINTHES
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CHOLINE
DIGESTIVE SYSTEM
DISEASES
DRUGS
ESTERS
GASTROINTESTINAL TRACT
HELMINTHS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INFECTIOUS DISEASES
INTESTINES
ISOTOPE APPLICATIONS
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MICE
MUSCLES
NEMATODES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASITIC DISEASES
PARASYMPATHOMIMETICS
PATHOGENESIS
QUATERNARY COMPOUNDS
RATS
RODENTS
SECRETION
SMALL INTESTINE
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRICHINELLA
TRICHINOSIS
TRITIUM COMPOUNDS
VERTEBRATES