Cross-linking of endothelin 1 and endothelin 3 to rat brain membranes: Identification of the putative receptor

Journal Article · Tue Jul 10 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00479a012· OSTI ID:6291318

Ambar, I; Kloog, Y; Sokolovsky, M ^[1]

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1. Tel Aviv Univ. (Israel)

Affinity-labeling experiments with {sup 125}I-endothelin derivatives using bifunctional cross-lining reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labeled a major component with a molecular mass of around 53,000. In the same tissues endothelin 3 specifically labeled, in addition to the 53,000 band, a band of molecular mass of 38,000. This result clearly indicates that in the brain the endothelin binding site resides within a polypeptide of apparent M{sub r} = 53,000. The possible presence of receptor subtypes is discussed with reference also to the reported identification of endothelin receptors in chick cardiac membrane and in rat mesangial cells.

• OSTI ID: 6291318
• Journal Information: Biochemistry; (USA), Vol. 29:27; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
CROSS-LINKING
PEPTIDES
MOLECULAR WEIGHT
VASOCONSTRICTORS
BRAIN
CEREBELLUM
ELECTROPHORESIS
IODINE 125
RATS
RECEPTORS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques