Continuous production of erythropoietin by an established human renal carcinoma cell line: development of the cell line

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.1.165· OSTI ID:6291214

Sherwood, J B; Shouval, D

Establishment of a stable, transformed human renal carcinoma cell line that produces erythropoietin in vitro and has maintained this function continuously since 1981 and for > 150 passages in monolayer culture was accomplished by transplantation of human renal clear cell carcinoma tissue from a patient with erythrocytosis into an immunosuppressed athymic mouse. In addition to its immunocrossreactivity with native human urinary erythropoietin, the tumor erythropoietin demonstrates biological activity in the in vitro mouse erythroid colony-forming unit assay and in tumor-bearing nude mice. The cloned renal carcinoma cell line has an abnormal human karyotype and has ultrastructural features characteristic of human renal clear cell carcinoma. This cell line provides a reproducible model system for the production of an erythropoietin-like material and for the study of its synthesis and secretion.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY
• OSTI ID: 6291214
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:1; ISSN PNASA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOMAS
CESIUM 137
CESIUM ISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
ERYTHROPOIETIN
GAMMA RADIATION
HORMONES
IMMUNOASSAY
IMMUNOLOGY
IMMUNOSUPPRESSION
IN VITRO
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MATERIALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
RADIATION EFFECTS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOINDUCTION
RADIOISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TUMOR CELLS
YEARS LIVING RADIOISOTOPES