Purification of the pyrazole-inducible cytochrome P-450 isozyme

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6290676

Palakodety, R; Clejan, L; Krikun, G; Feierman, D; Cederbaum, A I

The alcohol dehydrogenase inhibitor, pyrazole, appears to induce a cytochrome P-450 isozyme with properties similar to the ethanol-inducible P-450. The pyrazole-inducible P-450 isozyme was purified from the liver microsomes of rats treated with pyrazole essentially by the procedure of Ryan et al and also by chromatofocussing. The final preparation appeared homogenous by SDS-PAGE with an apparent molecular weight of 52,000, had a specific content of 11 nmoles P-450 per mg protein, showed very high activity of low K/sub m/ dimethylnitrosamine demethylase and produced a type II binding spectrum with dimethylsulfoxide. The enzyme was also active with aniline and aminopyrine as substrates. Pyrazole itself served as an excellent substrate with 4-hydroxy pyrazole being the product. An antibody against the pyrazole-inducible P-450 raised in chickens recognized a protein with mol.wt of about 52,000 in control microsomes. This band was highly enriched in microsomes from rats treated with pyrazole, 4-methyl-pyrazole, ethanol or acetone, but not phenobarbital or 3-methylcholanthrene. In summary, the pyrazole-inducible P-450 has been purified and appears to be identical in its catalytic and immunological properties to the alcohol-inducible P-450.

• Research Organization: Mount Sinai School of Medicine, New York, NY
• OSTI ID: 6290676
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETONE
ALCOHOLS
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AROMATICS
AZINES
AZOLES
BARBITURATES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DMSO
DRUGS
ELECTROPHORESIS
ENZYMES
ETHANOL
FRACTIONATION
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
HYPNOTICS AND SEDATIVES
KETONES
KINETICS
LIVER
MAMMALS
MICROSOMES
MIXED-FUNCTION OXIDASES
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PHENOBARBITAL
POLYCYCLIC AROMATIC HYDROCARBONS
PYRAZOLES
PYRIMIDINES
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SULFOXIDES
VERTEBRATES