Hepatic transformation of prostaglandin D2 to a new prostanoid, 9 alpha,11 beta-prostaglandin F2, that inhibits platelet aggregation and constricts blood vessels

Journal Article · Mon Nov 25 04:00:00 EST 1985 · J. Biol. Chem.; (United States)

OSTI ID:6290545

Pugliese, G; Spokas, E G; Marcinkiewicz, E; Wong, P Y

The metabolic transformation of tritium-labeled prostaglandin D2 ((TH)PGD2) was investigated in the isolated Tyrode's-perfused rabbit liver. One major product was isolated and identified in the perfusate as a new prostanoid. The structure of this metabolite was further confirmed by gas chromatography-mass spectrometry and chemical methods to be 9 alpha,11 beta,15-L-trihydroxyprosta-5-cis, 13-trans-dienoic acid, namely (9 alpha,11 beta-PGF2). This new prostanoid was found to be an inhibitor of platelet aggregation and to cause constriction of canine coronary artery strips. These results suggested that on passage through the hepatic circulation exogenous PGD2 is converted to 9 alpha,11 beta-PGF2, the latter having a biological profile which differs from that of PGD2 and PGF2 alpha.

• Research Organization: New York Medical College, Valhalla
• OSTI ID: 6290545
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 27; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTERIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
COAGULANTS
CORONARIES
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MATERIALS
METABOLISM
METABOLITES
MOLECULAR STRUCTURE
ORGANS
PEPTIDE HYDROLASES
PERFUSED ORGANS
PROSTAGLANDINS
RABBITS
SERINE PROTEINASES
THROMBIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOCONSTRICTION
VERTEBRATES