Improved indirect and direct radioimmunoassay for carcinoembryonic antigen. [/sup 125/I tracer technique]
Conference
·
· Clin. Chem.; (United States)
OSTI ID:6289728
Values obtained by the indirect and the direct assays for carcinoembryonic antigen by use of the CEA Roche method and reagents differ significantly. In 32 samples the latter method gave values almost twofold those obtained by the former. The mean ratio for direct/indirect was 1.9 +- 0.6. Analytical recovery experiments on samples to which known amounts of the antigen had been added showed a similar disparity. A simple modification of these two procedures, involving addition of normal human plasma (containing less than 2.0 ..mu..g of the antigen per liter) to standards, eliminated this discrepancy. In the indirect method, standards were added to normal human plasma, then extracted with perchloric acid before assay. In the direct assay normal human plasma instead of goat serum, was added to standards. With these modifications the analytical recoveries of added CEA by the indirect and direct methods were 87 +- 17% and 99.7 +- 8%, respectively, and the mean ratio between two methods was 1.2 +- 0.4.
- Research Organization:
- Cleveland Clinic Foundation, OH
- OSTI ID:
- 6289728
- Conference Information:
- Journal Name: Clin. Chem.; (United States) Journal Volume: 25:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GRAPHS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
MATHEMATICS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STANDARDS
STATISTICS
TRACER TECHNIQUES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GRAPHS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
MATHEMATICS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STANDARDS
STATISTICS
TRACER TECHNIQUES