Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure

Journal Article · Sat Oct 01 00:00:00 EDT 1983 · J. Nucl. Med.; (United States)

OSTI ID:5076808

Witherspoon, L R; Shuler, S E; Alyea, K; Husserl, F E

Heat inactivation has been proposed as an alternative to perchloric acid (PCA) precipitation for the extraction of carcinoembryonic antigen (CEA) from human plasma. A commercial RIA kit using heat inactivation was examined and results compared with those obtained with PCA precipitation. Adequate sensitivity (1.5 ..mu..g CEA/I plasma), satisfactory analytical recovery of CEA added to plasma, and dilutional linearity of samples found to have elevated CEA concentrations, were demonstrated for the heat-inactivation assay. Between-assay precision was better with the heat inactivation than with the PCA assay. Although the absolute concentration of CEA estimated after heat inactivation was consistently lower than that estimated after PCA extraction of plasma specimens, there was excellent correlation between results obtained with the two methods in colon cancer patients free of disease, colon cancer patients with residual or recurrent disease, patients with benign gastrointestinal disease, and in patients with chronic renal failure. The heat-inactivation assay is an excellent alternative to the PCA assay.

• Research Organization: Ochsner Clinic; Alton Ochsner Medical Foundation, New Orleans, LO
• OSTI ID: 5076808
• Journal Information: J. Nucl. Med.; (United States), Vol. 24:10
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOEMBRYONIC ANTIGEN
CALIBRATION STANDARDS
RADIOIMMUNOASSAY
HEAT
COMPARATIVE EVALUATIONS
PERCHLORIC ACID
DIGESTIVE SYSTEM DISEASES
EXTRACTION
INACTIVATION
KIDNEYS
LARGE INTESTINE
NEOPLASMS
PATIENTS
ANTIGENS
BODY
DIGESTIVE SYSTEM
DISEASES
ENERGY
GASTROINTESTINAL TRACT
HYDROGEN COMPOUNDS
INORGANIC ACIDS
INTESTINES
ISOTOPE APPLICATIONS
ORGANS
RADIOASSAY
SEPARATION PROCESSES
STANDARDS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics