Application of denaturing gradient gel electrophoresis to detect DNA sequence differences encoding apolipoprotein E isoforms
- Joslin Diabetes Center, Boston, MA (United States) Harvard Medical School, Boston, MA (United States)
Apolipoprotein E (apoE) plays an important role in plasma lipid metabolism. Three common isoforms of this protein have been identified by the isoelectric focusing method. In this report the authors describe a new method for distinguishing these isoforms. Their method employs PCR amplification of the DNA sequence of exon 4 in the apoE gene followed by denaturing gradient gel electrophoresis (DGGE) to distinguish its different melting characteristics. Identification of the ApoE isoforms through DNA melting behavior rather than protein charge differences eliminates the problems associated with isoelectric focusing and facilitates screening for additional mutations at the apoE locus. 12 refs., 2 figs.
- OSTI ID:
- 6287903
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 16:1; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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