Analysis of mutations using PCR and denaturing gradient gel electrophoresis

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180408· OSTI ID:5481739

Cariello, N F; Swenberg, J A ^[1]; DeBellis, A; Skopek, T R ^[2]

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1. Univ. of North Carolina, Chapel Hill (United States) Duke Univ., Durham, NC (United States)
2. Univ. of North Carolina, Chapel Hill (United States)

Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on primary sequence. Under the appropriate conditions, all base pair (bp) substitutions, frameshifts, and deletions less than about 10 bp can be resolved from the wild type sequence using DGGE. Polymerase chain reaction (PCR) permits facile amplification of a given region of the genome. The authors have combined PCR and DGGE to: (1) localize mutations in the X-linked human androgen receptor gene; (2) analyze thousands of thioguanine-resistant mutants simultaneously; (3) examine the fidelity of several DNA polymerases used in PCR.

• OSTI ID: 5481739
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:4; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANDROGENS
ANDROSTANES
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
CHROMOSOMES
DNA
DNA POLYMERASES
ELECTROPHORESIS
ENZYMES
GENE AMPLIFICATION
GENE MUTATIONS
GENETIC EFFECTS
HETEROCHROMOSOMES
HORMONES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
MOLECULAR BIOLOGY
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLATINUM COMPOUNDS
POLYMERASES
PROTEINS
STEROID HORMONES
STEROIDS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
X CHROMOSOME