Steady-state kinetic analysis of triacylglycerol delivery into mesenteric lymph
The output of triacylglycerol in chylomicrons can be increased 60% by prefeeding rats with a 20% fat diet or 110% by including phosphatidylcholine in a lipid infusion. The present study was designed to determine whether the increment was due to an expansion of the chylomicron triacylglycerol precursor pool or an increase in its fractional turnover rate. A steady-state kinetic model was established in rats receiving 135 mol glyceryl trioleate/h. The decay in specific activity of triacylglycerol after removal of radiolabeled glyceryl trioleate from the duodenal infusate was followed for 4 h and analyzed by the SAAM 23 program. It was found that the fractional turnover rate of the chylomicron precursor pool remained the same in each experimental condition. However, the pool was found to expand in direct proportion to the chylomicron triacylglycerol output. Functionally the infused (TH)glyceride-glycerol and tri( UC)oleate behaved the same in lymph chylomicrons and was 90% of infusate specific activity. In summary, these data suggest that increases in chylomicron triacylglycerol output are dependent on the size of the mucosal precursor pool and the monoacylglycerol acyltransferase synthetic pathway for its triacylglycerol.
- Research Organization:
- Duke Univ. of Medical Center, Durham, NC
- OSTI ID:
- 6285047
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ALCOHOLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ESTERS
GASTROINTESTINAL TRACT
GLYCEROL
HYDROXY COMPOUNDS
INTESTINES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LYMPH
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
OILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
PHOSPHOLIPIDS
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SMALL INTESTINE
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRIGLYCERIDES
TRIOLEIN
TRITIUM COMPOUNDS
VERTEBRATES