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Detection of benzo(a)pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic hydrocarbons, including benzo(a)pyrene (B(a)P), and are at increased risk of lung cancer. Since B(a)P is enzymatically activated to 7 beta,8 alpha-dihydroxy(9 alpha, 10 alpha)epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (B(a)PDE) that forms adducts with DNA, the presence of these adducts was measured in DNA from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry and enzyme radioimmunoassay. Approximately two-thirds of the workers had detectable levels of B(a)PDE-DNA adducts. Antibodies to the DNA adducts were also found in the serum of 27% of the workers. B(a)PDE-DNA adducts were not detectable in lymphocytes and antibodies to the adducts were not detected in sera from a control group of nonsmoking laboratory workers. DNA adducts and/or antibodies to the adducts indicate exposure to B(a)P and its metabolic activation to the carcinogenic metabolite that covalently binds to and damages DNA. Detection of adducts and antibodies to them may also be useful as internal dosimeters of the pathobiological effective doses of chemical carcinogens.

Research Organization:
National Cancer Institute, Bethesda, MD
OSTI ID:
6284724
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 19; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL CELLS
ANTIBODIES
AROMATICS
BENZOPYRENE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD CHEMISTRY
BODY FLUIDS
CARBONACEOUS MATERIALS
COAL
COKE
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DNA
DNA ADDUCTS
EMISSION SPECTROSCOPY
ENERGY SOURCES
FLUORESCENCE SPECTROSCOPY
FOSSIL FUELS
FUELS
HYDROCARBONS
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NUCLEIC ACIDS
OCCUPATIONAL DISEASES
ORGANIC COMPOUNDS
OVEN COKE
PERSONNEL
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SOMATIC CELLS
SPECTROSCOPY
TRACER TECHNIQUES