Determination of polycyclic aromatic hydrocarbons in the urine, benzo(a)pyrene diol epoxide-DNA adducts in lymphocyte DNA, and antibodies to the adducts in sera from coke oven workers exposed to measured amounts of polycyclic aromatic hydrocarbons in the work atmosphere

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Cancer Res.; (United States)

OSTI ID:5142768

Haugen, A; Becher, G; Benestad, C; Vahakangas, K; Trivers, G E; Newman, M J; Harris, C C

Workers in coke oven plants have a higher incidence of lung cancer than the general population. They are exposed to a variety of chemicals, in particular the polycyclic aromatic hydrocarbons (PAH), including benzo(a)pyrene. To evaluate the genotoxic effects of PAH exposure, air samples and urine samples were analyzed for PAH by capillary gas chromatography and high-performance liquid chromatography, respectively. Since benzo(a)pyrene is activated to 7 beta,8 alpha-dihydroxy-(9 alpha,10 alpha)-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE) and binds to DNA, we have used ultrasensitive enzymatic radioimmunoassay and synchronous fluorescence spectrophotometry to measure BPDE-DNA adducts in lymphocyte DNA. The results show that workers were exposed to high concentrations of atmospheric PAH. However, the mean PAH exposure levels are reduced 60% when the workers wore masks during work. When compared to exposure levels, the urinary excretion of PAH was relatively low. Approximately one-third of the workers had detectable putative BPDE-DNA adducts in lymphocytes by ultrasensitive enzymatic radioimmunoassay, and 10% of the samples had emission peaks at 379 nm by synchronous fluorescence spectrophotometry. The four most positive samples were the same in both of the assays. Antibodies to an epitope(s) on BPDE-DNA were found in the sera of approximately one-third of the workers. Detection of DNA adducts and antibodies to these adducts are internal indicators of exposure to benzo(a)pyrene.

• Research Organization: National Institute of Public Health, Oslo, Norway
• OSTI ID: 5142768
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 8; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AIR
ANIMAL CELLS
ANTIBODIES
AROMATICS
BENZOPYRENE
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY FLUIDS
CHEMICAL ANALYSIS
COKE OVENS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DNA
DNA ADDUCTS
EPOXIDES
FLUIDS
GASES
HYDROCARBONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONITORING
NUCLEIC ACIDS
OCCUPATIONAL EXPOSURE
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PERSONNEL
POLYCYCLIC AROMATIC HYDROCARBONS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SOMATIC CELLS
TRACER TECHNIQUES
URINE
WASTES