Islet cell thymidine kinase activity as indicator of islet cell proliferation in rat pancreas

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Diabetes; (USA)

DOI:https://doi.org/10.2337/diabetes.39.1.70· OSTI ID:6284045

Swenne, I ^[1]

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1. Biomedicum, Uppsala (Sweden)

The activity of thymidine kinase in homogenates of isolated rat islets of Langerhans was measured and correlated with the DNA replicatory activity of the islet cells. Adult and fetal rat islets were cultured in medium with 2.7 or 16.7 mM glucose or 16.7 mM glucose and 1 microgram/ml human growth hormone. In both types of islets, 16.7 mM glucose doubled (3H)thymidine incorporation compared with 2.7 mM glucose, and the addition of growth hormone caused a further increase in DNA replication. TK activity in the islets showed similar changes in response to glucose and growth hormone. The correlation between (3H)thymidine incorporation and TK activity was thus highly significant. Cell-cycle analysis of cultured fetal rat islets showed that TK activity was preferentially expressed during the S phase of the cell cycle. TK activity of freshly isolated islets declined with the age of the animal. In pancreatic sections, the islet cell autoradiographic labeling index after (3H)thymidine administration in vivo likewise declined with age and was correlated with the TK activity in freshly isolated islets. It is suggested that measurements of islet TK activity can be used as index of islet cell proliferation; this method has the distinct advantage of avoiding the cumbersome procedure of preparing and scoring autoradiograms.

• OSTI ID: 6284045
• Journal Information: Diabetes; (USA), Journal Name: Diabetes; (USA) Vol. 39:1; ISSN 0012-1797; ISSN DIAEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL MARKERS
BODY
CARBOHYDRATES
CELL CYCLE
CELL PROLIFERATION
DIGESTIVE SYSTEM
DNA REPLICATION
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
GLANDS
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROGEN COMPOUNDS
MAMMALS
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PANCREAS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SACCHARIDES
THYMIDINE
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES