Hormonal regulation of Na -K -ATPase in cultured epithelial cells

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6281110

Johnson, J P; Jones, D; Wiesmann, W P

Aldosterone and insulin stimulate Na transport through mechanisms involving protein synthesis. Na -K -ATPase has been implicated in the action of both hormones. The authors examined the effect of aldosterone and insulin on Na -K -ATPase in epithelial cells in culture derived from toad urinary bladder (TB6C) and toad kidney (A6). Aldosterone, but not insulin, increases short-circuit current (I/sub sc/) in TB6C cells. Aldosterone increases Na -K -(TSP)ATPase activity after 18 h of incubation, but no effect can be seen at 3 and 6 h. Amiloride, which inhibits aldosterone-induced increases in I/sub sc/, has no effect on either basal or aldosterone stimulated enzyme activity. Both aldosterone and insulin increase I/sub sc/ in A6 cells and when added together are synergistic. Aldosterone stimulates enzyme activity in A6 cells, but insulin alone has no effect. However, aldosterone and insulin together stimulate enzyme activity more than aldosterone alone. It appears that stimulation of Na -K -ATPase activity is involved in aldosterone action in both cell lines but does not appear to be due to increased Na entry, since enhanced enzyme activity is not inhibited by amiloride. In contrast, insulin alone has no direct effect on Na -K -ATPase, although the increased enzyme activity following both agents in combination may explain their synergism on I/sub sc/.

• Research Organization: Walter Reed Army Institute of Research, Washington, DC
• OSTI ID: 6281110
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ALKALI METAL COMPOUNDS
AMPHIBIANS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BLADDER
BODY
CHEMISTRY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
IN VITRO
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KIDNEYS
LIGHT NUCLEI
MINERALOCORTICOIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
RADIOISOTOPES
SODIUM COMPOUNDS
STEROID HORMONES
STEROIDS
TISSUES
TOADS
TRACER TECHNIQUES
URINARY TRACT
VERTEBRATES