Effect of putrescine on oxyntic gland and colonic mucosal growth in rats

Journal Article · Mon Aug 24 04:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90683-7· OSTI ID:6279593

Majumdar, A P.N.; Johnson, L R

The effect of putrescine on oxyntic gland and colonic mucosal growth was studied by measuring the rate of (/sup 3/H)-thymidine incorporation into mucosal DNA in vitro (DNA synthesis) and DNA, RNA and protein content of the mucosa following intramuscular injections of the compound (50 ..mu..moles/100g). Saline injected animals served as controls. Multiple injections of putrescine during a 2-day fast produced a significant enhancement of mucosal DNA synthesis in oxyntic gland and colonic mucosa, with no apparent change in DNA, RNA or protein content in either of the tissues, compared to the corresponding saline-controls, when measurements were made 12-14 h after the last injection. However, when the animals were killed after 4 days, DNA, RNA and protein content of oxyntic gland mucosa, and DNA and protein content of colonic mucosa were found to be significantly higher than in the respective saline-controls. The authors conclude that putrescine, taken up from the blood, can stimulate growth of gastrointestinal mucosa. 26 references, 2 figures.

• Research Organization: Univ. of Texas Medical School, Houston
• OSTI ID: 6279593
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:8; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BODY
CELL PROLIFERATION
DATA
DIGESTIVE SYSTEM
DNA
DNA REPLICATION
EXPERIMENTAL DATA
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INFORMATION
INTESTINES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LARGE INTESTINE
MAMMALS
MEMBRANES
MUCOUS MEMBRANES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PUTRESCINE
PYRIMIDINES
RATS
REACTION KINETICS
RIBOSIDES
RNA
RODENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES