Effects of ozone on transepithelial potential of mouse trachea

Journal Article · Thu Jul 01 04:00:00 EDT 1993 · American Journal of Physiology; (United States)

OSTI ID:6279362

Takahashi, M; Kleeberger, S R; Croxton, T L ^[1]

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1. Johns Hopkins Medical Institutions, Baltimore, MD (United States)

The effects of ozone on tracheal electrical potential were investigated in inbred strains of mice that are differentially susceptible to ozone-induced inflammation. In male mice (9-13 wk), a tracheostomy was made under pentobarbital anesthesia for spontaneous breathing and tracheal potential was measured in the cephalad portion of the bisected trachea using Hanks' salt/agar-capped KCl bridges connected to a pair of calomel half cells. The mean tracheal potentials of five different strains of mice (C3H/HeJ, DBA/2J, C57BL/6J, BALB/cJ, and 129/J) were approximately 10 mV (lumen negative) with no significant interstrain difference. Amiloride reduced mouse tracheal potentials by approximately 70% in both C3H/HeJ and C57BL/6J mice, indicating that sodium absorption is the predominant ion transport across this tissue. Relative to air-exposed controls, acute ozone exposure (2 ppm for 3 h) significantly attenuated tracheal potential of inflammation-susceptible C57BL/6J mice by approximately 50% at 6 h and 40% at 24 h postexposure but had no effect immediately after exposure. The mean tracheal potential of C3H/HeJ mice was not changed by ozone. The differential effect of acute ozone exposure on tracheal potential in C57BL/6J and C3H/HeJ mice is consistent with differential susceptibility to ozone-induced increases in epithelial permeability in these strains.

• OSTI ID: 6279362
• Journal Information: American Journal of Physiology; (United States), Journal Name: American Journal of Physiology; (United States) Vol. 265:1 Pt 1; ISSN 0002-9513; ISSN AJPHAP
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
ELECTROPHYSIOLOGY
EPITHELIUM
INFLAMMATION
MAMMALS
MICE
OZONE
PATHOLOGICAL CHANGES
PHYSIOLOGY
RESPIRATORY SYSTEM
RODENTS
SYMPTOMS
TISSUES
TRACHEA
VERTEBRATES