Susceptibility to ozone-induced inflammation. II. Separate loci control responses to acute and subacute exposures

Journal Article · Fri Jan 01 04:00:00 EST 1993 · American Journal of Physiology; (United States)

OSTI ID:7061312

Kleeberger, S R; Levitt, R C; Zhang, L Y ^[1]

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1. Johns Hopkins Medical Institutions, Baltimore, MD (United States)

We demonstrated previously that inbred strains of mice are differentially susceptible to acute (3 h) and subacute (48 h) exposures to 2 parts per million (ppm) ozone (O3) and 0.30 ppm O3, respectively. Genetic studies with O3-resistant C3H/HeJ and O3-susceptible C57BL/6J strains have indicated that susceptibility to each of these O3 exposures is under Mendelian (single gene) control. In the present study, we hypothesized that the same gene controls susceptibility to the airway inflammatory responses to 2 ppm and 0.30 ppm O3 exposures. To test this hypothesis, airway inflammation was induced in 10 BXH and 16 BXD recombinant inbred (RI) strains of mice by acute as well as subacute O3 exposures. Airway inflammation was assessed by counting the number of polymorphonuclear leukocytes (PMNs) in bronchoalveolar lavage (BAL) returns obtained immediately after 48-h subacute exposure to 0.30 ppm O3, or 6 h after 3 h acute exposure to 2 ppm O3. Each RI strain was classified as susceptible or resistant to each exposure, based on a comparison of mean numbers of PMNs with those of the respective progenitor strains. For each RI set, a phenotypic strain distribution pattern (SDP) was thus derived for each exposure regimen, and the SDPs were then compared for concordance. Among the BXH RI strains, 4 of 10 responded discordantly to the two exposures: 3 were susceptible to acute exposure and resistant to subacute exposure, whereas 1 was conversely susceptible. Among the BXD RI strains, 4 of 16 were discordant: 1 was susceptible to acute exposure, and resistant to subacute exposure, whereas 3 were conversely susceptible.

• OSTI ID: 7061312
• Journal Information: American Journal of Physiology; (United States), Journal Name: American Journal of Physiology; (United States) Vol. 264:1 Pt 1; ISSN AJPHAP; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
CONTROL
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ENVIRONMENTAL EXPOSURE
GENETIC CONTROL
HYBRIDIZATION
INFLAMMATION
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
OZONE
PATHOLOGICAL CHANGES
PEST CONTROL
PHAGOCYTES
PNEUMONIA
PROGENY
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RESPONSE MODIFYING FACTORS
RODENTS
SOMATIC CELLS
STRAINS
SYMPTOMS
VERTEBRATES