Metabolism of methanol in acetogenic bacteria
Thesis/Dissertation
·
OSTI ID:6277212
Acetogens can grown on methanol in the presence of a cosubstrate that is more oxidized than methanol. Three mol of acetate is formed from 4 mol methanol and 2 mol CO/sub 2/. One mol of methanol is oxidized to CO/sub 2/. The levels of the tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and corrinoids indicate the presence of the acetyl CoA pathway when growing on methanol. The acetyl-CoA pathway of acetate synthesis as presently understood does not include methanol as a substrate. It is demonstrated that methanol is oxidized to formaldehyde and then to formate by a methanol dehydrogenase. It is also possible that the methyl group of methanol is transferred directly to either a corrinoid-type enzyme, or tetrahydrofolate. When cells of C. thermoautotrophicum are grown on /sup 14/CO/sub 2/, acetate becomes labeled in both carbons with a ratio /sup 14/CH/sub 3///sup 14/COOH of 0.7. In addition, methanol gets labeled. When cells are grown on /sup 14/CH/sub 3/OH, label appears in both acetate carbons with a ratio of 3.3, and also appears in CO/sub 2/. Thus methanol is preferentially incorporated into the methyl group of acetate, whereas CO/sub 2/ is the preferred source of the carboxyl carbon.
- Research Organization:
- Georgia Univ., Athens (USA)
- OSTI ID:
- 6277212
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ALCOHOLS
ALDEHYDES
BACTERIA
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHALCOGENIDES
ENZYME ACTIVITY
ENZYMES
FORMALDEHYDE
FORMIC ACID
GROWTH
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANOL
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
TRACER TECHNIQUES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ALCOHOLS
ALDEHYDES
BACTERIA
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHALCOGENIDES
ENZYME ACTIVITY
ENZYMES
FORMALDEHYDE
FORMIC ACID
GROWTH
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANOL
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
TRACER TECHNIQUES