Application of the micro-forward mutation assay to assess mutagenicity of airborne particulates in indoor

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6275358

Takagi, Y; Goto, S; Murata, M; Matsushita, H; Lewtas, J

A validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9 mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is greater by ten times or more than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by 30 cu m-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6275358
• Report Number(s): PB-91-109231/XAB; EPA-600/J-88/542
• Resource Relation: Other Information: Text in Japanese and English. Pub. in Jnl. Japan Soc. Air Pollut., Vol. 23, 24-31(1988). Portions of this document are not fully legible
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PARTICULATES
MUTAGEN SCREENING
INDOOR AIR POLLUTION
PARTICLE SIZE
SALMONELLA TYPHIMURIUM
SAMPLING
AIR POLLUTION
BACTERIA
MICROORGANISMS
PARTICLES
POLLUTION
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SCREENING
SIZE
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