Biological and chemical methodologies for assessing human exposure to airborne mutagens indoors

Technical Report · Mon Jan 01 00:00:00 EST 1990

OSTI ID:5990970

Matsushita, H; Goto, S; Endo, O; Koyano, M; Tanabe, K

Two highly sensitive methods have been developed and applied to the monitoring of personal exposure to airborne mutagens and carcinogenic polycyclic aromatic hydrocarbons (PAH). The automatic method for PAH analysis consists of ultrasonic extraction and multicolumn HPLC/computer controlled spectrofluorometric detection, by which 7 PAHs in indoor and/or personal particulate samples were analyzed. Concentration of PAHs, e.g., benzo(a)pyrene, indoors increased remarkably by smoking in a poorly ventilated room. The second method, an ultra microsuspension forward mutation assay using Salmonella typhimurium TM677, is about 100 times more sensitive than the ordinary Ames mutation bioassay. Mutagenicity of airborne particles collected by personal sampling was determined by this method. Personal exposures to ETS were greatest for smokers, followed by passive smokers and lowest for nonsmokers suspension.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 5990970
• Report Number(s): PB-91-133025/XAB; EPA-600/D-90/162
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
POLYCYCLIC AROMATIC HYDROCARBONS
AIR POLLUTION MONITORING
MUTAGEN SCREENING
ECOLOGICAL CONCENTRATION
INDOOR AIR POLLUTION
LIQUID COLUMN CHROMATOGRAPHY
SALMONELLA TYPHIMURIUM
AIR POLLUTION
AROMATICS
BACTERIA
CHROMATOGRAPHY
HYDROCARBONS
MICROORGANISMS
ORGANIC COMPOUNDS
POLLUTION
SALMONELLA
SCREENING
SEPARATION PROCESSES
540120* - Environment
Atmospheric- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology