Biological and chemical methodologies for assessing human exposure to airborne mutagens indoors
Two highly sensitive methods have been developed and applied to the monitoring of personal exposure to airborne mutagens and carcinogenic polycyclic aromatic hydrocarbons (PAH). The automatic method for PAH analysis consists of ultrasonic extraction and multicolumn HPLC/computer controlled spectrofluorometric detection, by which 7 PAHs in indoor and/or personal particulate samples were analyzed. Concentration of PAHs, e.g., benzo(a)pyrene, indoors increased remarkably by smoking in a poorly ventilated room. The second method, an ultra microsuspension forward mutation assay using Salmonella typhimurium TM677, is about 100 times more sensitive than the ordinary Ames mutation bioassay. Mutagenicity of airborne particles collected by personal sampling was determined by this method. Personal exposures to ETS were greatest for smokers, followed by passive smokers and lowest for nonsmokers suspension.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 5990970
- Report Number(s):
- PB-91-133025/XAB; EPA--600/D-90/162
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540120* -- Environment
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AIR POLLUTION MONITORING
AROMATICS
BACTERIA
CHROMATOGRAPHY
ECOLOGICAL CONCENTRATION
HYDROCARBONS
INDOOR AIR POLLUTION
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
MUTAGEN SCREENING
ORGANIC COMPOUNDS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES