Purification of Xenopus laevis mitochondrial RNA polymerase and identification of a dissociable factor required for specific transcription
Journal Article
·
· Mol. Cell. Biol.; (United States)
The Xenopus laevis mitochondrial RNA (mtRNA) polymerase was purified to near homogeneity with an overall yield approaching 50%. The major polypeptides in the final fraction were a doublet of proteins of approximately 140 kilodaltons that copurified with the mtRNA polymerase activity. It appeared likely that the smaller polypeptide is a breakdown product of the larger one. The highly purified polymerase was active in nonspecific transcription but required a dissociable factor for specific transcription of X. laevis mtDNA. The factor could be resolved from mtRNA polymerase by hydrophobic chromatography and had a sedimentation coefficient of 3.0 S. The transcription factor eluted from both the hydrophobic column and a Mono Q anion-exchange column as a single symmetrical peak. The mtRNA polymerase and this factor together are necessary and sufficient for active transcription from four promoters located in a noncoding region of the mtDNA genome between the gene for tRNA/sup Phe/ and the displacement loop.
- Research Organization:
- Dept. of Pharmacology and Graduate Program in Genetics, State Univ. of New York at Stony Brook, Stony Brook, NY (US)
- OSTI ID:
- 6272689
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:7; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL FUNCTIONS
CELL CONSTITUENTS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
FROGS
FUNCTIONS
GENE OPERONS
GENES
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
MITOCHONDRIA
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RNA
RNA POLYMERASES
SEPARATION PROCESSES
TRANSCRIPTION FACTORS
TRANSFER RNA
TRANSFERASES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL FUNCTIONS
CELL CONSTITUENTS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
FROGS
FUNCTIONS
GENE OPERONS
GENES
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
MITOCHONDRIA
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RNA
RNA POLYMERASES
SEPARATION PROCESSES
TRANSCRIPTION FACTORS
TRANSFER RNA
TRANSFERASES
VERTEBRATES