DNA polymerase gamma from Xenopus laevis. I. The identification of a high molecular weight catalytic subunit by a novel DNA polymerase photolabeling procedure

Journal Article · Mon Dec 25 04:00:00 EST 1989 · Journal of Biological Chemistry; (USA)

OSTI ID:7105872

Insdorf, N F; Bogenhagen, D F ^[1]

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1. State Univ. of New York, Stony Brook (USA)

DNA polymerase gamma has been purified over 10,000-fold from mitochondria of Xenopus laevis ovaries. We have developed a novel technique which specifically photolabels DNA polymerases. This procedure, the DNA polymerase trap, was used to identify a catalytic subunit of 140,000 Da from X. laevis DNA polymerase gamma. Additional catalytically active polypeptides of 100,000 and 55,000 Da were identified in the highly purified enzyme. These appear to be products of degradation of the 140,000-Da subunit. The DNA polymerase trap, which does not require large amounts of enzyme or renaturation from sodium dodecyl sulfate, is an alternative to the classic activity gel.

• OSTI ID: 7105872
• Journal Information: Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 264:36; ISSN JBCHA; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BODY
CENTRIFUGATION
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DNA POLYMERASES
ELECTROPHORESIS
ENZYMES
FEMALE GENITALS
FRACTIONATION
FROGS
GERM CELLS
GONADS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEOTIDYLTRANSFERASES
OOCYTES
ORGANS
OVARIES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
SEPARATION PROCESSES
TRANSFERASES
ULTRACENTRIFUGATION
VERTEBRATES