ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene
Journal Article
·
· Mol. Cell. Biol.; (United States)
Copper resistance in Saccharomyces cerevisiae is mediated, in large part, by the CUP1 locus, which encodes a low-molecular-weight, cysteine-rich metal-binding protein. Expression of the CUP1 gene is regulated at the level of transcriptional induction in response to high environmental copper levels. This report describes the isolation of a yeast mutant, ace1-1, which is defective in the activation of CUP1 expression upon exposure to exogeneous copper. The ace1-1 mutation is recessive and lies in a genetic element that encodes a trans-acting CUP1 regulatory factor. The wild-type ACE1 gene was isolated by in vivo complementation and restores copper inducibility of CUP1 expression and copper resistance to the otherwise copper-sensitive ace1-1 mutant. Linkage analysis and gene deletion experiments verified that this gene represents the authentic ACE 1 locus. ACE1 maps to the left arm of chromosome VII, 9 centimorgans centromere distal to lys5. The ACE 1 gene appears to play a direct or indirect positive role in activation of CUP1 expression in response to elevated copper concentrations.
- Research Organization:
- Dept. of Biological Chemistry, The Univ. of Michigan Medical School, Ann Arbor, MI (US)
- OSTI ID:
- 6272606
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:7; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
AMINO ACIDS
BIOLOGICAL ADAPTATION
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHROMOSOME LOSSES
CHROMOSOMES
COPPER
CYSTEINE
DATA
ELEMENTS
EUMYCOTA
EXPERIMENTAL DATA
FUNGI
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
INFORMATION
LOSSES
MAPPING
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICROORGANISMS
MUTATIONS
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTYPE
PLANTS
PROTEINS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
THIOLS
TRANSCRIPTION
TRANSITION ELEMENTS
YEASTS
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
AMINO ACIDS
BIOLOGICAL ADAPTATION
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHROMOSOME LOSSES
CHROMOSOMES
COPPER
CYSTEINE
DATA
ELEMENTS
EUMYCOTA
EXPERIMENTAL DATA
FUNGI
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
INFORMATION
LOSSES
MAPPING
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICROORGANISMS
MUTATIONS
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTYPE
PLANTS
PROTEINS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
THIOLS
TRANSCRIPTION
TRANSITION ELEMENTS
YEASTS