Use of HPLC for the detection of iron chelators in cultures of bacteria, fungi, and algae. [E. coli; Bacillus megaterium; Ustilago sphaerogena; Anabaena flos-aqua]
Conference
·
· Journal of Phycology; (USA)
OSTI ID:6271918
- State Univ. of New York, Syracuse (USA)
Iron is essential for the growth of living cells. To meet biochemical needs, microorganisms, including algae, produce high affinity chelators termed siderophores. These compounds solubilize Fe and increase its bioavailability. We have developed a new method to study siderophore formation in cultured and natural environments. Based on the fact siderophores tightly bind 55-Fe, the radioactive complexes can be separated by HPLC using an inert PRP-1 column and detected by scintillation counting. This method cleanly resolves several known siderophores, including ferrichrome A, ferrichrome, desferal, and rhodotorulic acid. The optimization of the method and its use for analysis of siderophore formation in bacteria (E. coli, and Bacillus megaterium), fungi (Ustilago sphaerogena), and cyanobacteria (Anabaena flos-aqua UTEX 1444 and Anabaena sp. ATCC 27898) will be presented.
- OSTI ID:
- 6271918
- Report Number(s):
- CONF-9006266--
- Conference Information:
- Journal Name: Journal of Phycology; (USA) Journal Volume: 26:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL AVAILABILITY
CHELATING AGENTS
CHROMATOGRAPHY
COUNTING TECHNIQUES
CYANOBACTERIA
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FUNGI
INTERMEDIATE MASS NUCLEI
IRON
IRON 55
IRON ISOTOPES
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
METALS
MICROORGANISMS
NUCLEI
PHYSIOLOGY
PLANTS
RADIOISOTOPES
SCINTILLATION COUNTING
SEPARATION PROCESSES
TRANSITION ELEMENTS
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL AVAILABILITY
CHELATING AGENTS
CHROMATOGRAPHY
COUNTING TECHNIQUES
CYANOBACTERIA
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FUNGI
INTERMEDIATE MASS NUCLEI
IRON
IRON 55
IRON ISOTOPES
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
METALS
MICROORGANISMS
NUCLEI
PHYSIOLOGY
PLANTS
RADIOISOTOPES
SCINTILLATION COUNTING
SEPARATION PROCESSES
TRANSITION ELEMENTS
YEARS LIVING RADIOISOTOPES