Synthesis of tritium labeled Ac-(Nle/sup 4/, D-Phe/sup 7/)-. cap alpha. -MSH/sub 4-11/-NH/sub 2/: a superpotent melanotropin with prolonged biological activity
Journal Article
·
· Life Sci.; (United States)
Ac-(Nle/sup 4/, D-Phe/sup 7/)-..cap alpha..-MSH/sub 4-11/-NH/sub 2/ an octapeptide, is a melanotropin analogue (Ac-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-NH/sub 2/), which is a superpotent agonist of frog and lizard skin melanocytes and mouse S 91 (Cloudman) melanoma cells. This melanotropin possesses ultraprolonged activity on melanocytes, both in vitro and in vivo, and the peptide is resistant to inactivation by serum enzymes. The tritium-labeled congener was prepared by direct incorporation of (/sup 3/H)-labeled norleucine into the peptide. The melanotropic activity of the labeled peptide is identical to the unlabeled analogue. This labeled peptide should be useful for studies on the localization and characterization of melanotropin receptors.
- Research Organization:
- Univ. of Arizona, Tucson
- OSTI ID:
- 6271768
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 34:10; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
FROGS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIZARDS
MAMMALS
MELANIN
MICE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PIGMENTS
PITUITARY HORMONES
RADIOASSAY
RECEPTORS
REPTILES
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
FROGS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIZARDS
MAMMALS
MELANIN
MICE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PIGMENTS
PITUITARY HORMONES
RADIOASSAY
RECEPTORS
REPTILES
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES