Species variance in the metabolic activation of polycyclic hydrocarbons

Journal Article · Fri Jan 01 04:00:00 EST 1982 · UCLA Symp. Mol. Cell. Biol., New Ser.; (United States)

OSTI ID:6271541

Selkirk, J K; MacLeod, M C; Moore, C J; Mansfield, B K; Nikbakht, A; Dearstone, K

Polycyclic hydrocarbons are metabolized to a series of oxygenated derivatives consisting of dihydrodiols, phenols, and quinones. A comparison of cell-free and intact cell systems shows considerable variation for benzo(a)pyrene metabolism depending upon the presence or absence of cytoplasmic transferases for conjugation of these hydroxylated derivatives. Comparative metabolism studies, including microsomes and intact cells from rat, mouse, hamster, and humans yield identical qualitative metabolite profiles with significant quantitative variation. Macromolecular studies of benzo(a)pyrene diol-epoxides in hamster cells show specific binding of 7,8-diol-9,10-epoxide to DNA and 9,10-diol-7,8-epoxide to selected nuclear proteins. A comparative analysis of sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis suggests a unique specificity for regions of the benzo(a)pyrene molecule at the nuclear level. In addition, studies of carcinogenic 2-hydroxy-benzo(a)pyrene in cell culture were studied. A diphenol was isolated and characterized as the major product in embryonic fibroblasts. A second minor metabolite was observed. The ratio of these two metabolites was reversed for epidermal cells.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6271541
• Journal Information: UCLA Symp. Mol. Cell. Biol., New Ser.; (United States), Journal Name: UCLA Symp. Mol. Cell. Biol., New Ser.; (United States); ISSN USMBD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL PATHWAYS
BODY
CELL CONSTITUENTS
CHEMICAL BONDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DNA
ELECTROPHORESIS
ENZYMES
EPOXIDES
FIBROBLASTS
GLANDS
HAMSTERS
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
MAN
METABOLIC ACTIVATION
METABOLISM
METABOLITES
MICE
MICROSOMES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PRIMATES
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SPECIFICITY
TRANSFERASES
VERTEBRATES