Dosimetry of PAH skin carcinogenesis: covalent binding of benzo(a)pyrene to mouse epidermal DNA
Benzo(a)pyrene (BaP) is metabolized to the chemically reactive anti and syn isomers of the 7,8-diol-9,10-epoxides of BaP (BPDE) which bind covalently to DNA to form DNA/BPDE complexes. Tetrols liberated from the DNA/BPDE complex by acid hydrolysis are easily quantified by h.p.l.c. using fluorescence detection. This approach allows femtomole amounts of BPDE associated with the DNA isolated from a single mouse to be detected using conventional instrumentation. The usefulness of this technique to estimate the interaction of BaP with DNA of mouse skin, both in the intact animal and in organ culture, was investigated. With mouse skin in organ culture it could be demonstrated that: (1) upon a single topical application of 5 ..mu..g of BaP, binding to DNA occurred via BPDE at a linear rate for up to 65 h, (2) the amount of binding was dose dependent at concentrations of BaP <10 ..mu..g.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6267656
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:2; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Metabolic activation of benzo(a)pyrene in SENCAR and BALB/c mouse embryo cell cultures
Surface-enhanced Raman analysis of Benzo(A)Pyrene-DNA adducts on silver-coated cellulose substrates
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
AROMATICS
BENZOPYRENE
BODY
CHEMICAL BONDS
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
EPIDERMIS
EPITHELIUM
HYDROCARBONS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLIC ACTIVATION
METABOLITES
MICE
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
RODENTS
SEPARATION PROCESSES
SKIN
STEREOCHEMISTRY
TISSUE CULTURES
TISSUES
VERTEBRATES