Restoration of viability to an Escherichia coli mutant deficient in the 5'. -->. 3' exonuclease of DNA polymerase I
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6265346
An Escherichia coli polA(Ex) mutant that is usually inviable at restrictive temperatures (43/sup 0/C) was found to grow normally at 43/sup 0/C when incubated in the presence of a membrane-containing fraction prepared from E. coli. This membrane fraction causes anaerobic conditions that are necessary but not sufficient for restoration of viability since some component present in the membrane fraction is also required for colony formation at 43/sup 0/C. The accumulation of small DNA fragments typical of aerobic growth of the polA(Ex) mutant was also seen under anaerobic conditions. The polA(Ex) strain was also much more sensitive than the isogenic wild-type strain to hydrogen peroxide.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6265346
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Journal Article
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Sun Dec 31 23:00:00 EST 1978
· J. Bacteriol.; (United States)
·
OSTI ID:6367374
DNA repair in DNA-polymerase-deficient mutants of Escherichia coli
Conference
·
Tue Dec 31 23:00:00 EST 1974
·
OSTI ID:4125893
Deoxyribonucleic acid repair in Escherichia coli mutants deficient in the 5'----3' exonuclease activity of deoxyribonucleic acid polymerase I and exonuclease VII
Journal Article
·
Sun May 01 00:00:00 EDT 1977
· J. Bacteriol.; (United States)
·
OSTI ID:5617916
Related Subjects
550400 -- Genetics
550700* -- Microbiology
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANAEROBIC CONDITIONS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
DNA
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RECOVERY
SENSITIVITY
TEMPERATURE EFFECTS
TRANSFERASES
550700* -- Microbiology
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANAEROBIC CONDITIONS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
DNA
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RECOVERY
SENSITIVITY
TEMPERATURE EFFECTS
TRANSFERASES