Genetic linkage map of 46 DNA markers on human chromosome 16

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5754· OSTI ID:6263382

Keith, T P; Green, P; Brown, V A; Phipps, P; Bricker, A; Falls, K; Rediker, K S; Powers, J A; Hogan, C; Nelson, C; Knowlton, R; Donis-Keller, H ^[1]; Reeders, S T ^[2]

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1. Collaborative Research Inc., Bedford, MA (USA)
2. John Radcliffe Hospital, Oxford (England)

The authors have constructed a genetic linkage map of human chromosome 16 based on 46 DNA markers that detect restriction fragment length polymorphisms. Segregation data were collected on a set of multigenerational families provided by the Centre d'Etude du Polymorphisme Humain, and maps were constructed using recently developed multipoint analysis techniques. The map spans 115 centimorgans (cM) in males and 193 cM in females. Over much of the chromosome there is a significantly higher frequency of recombination in females than males. Near the {alpha}-globin locus on the distal part of the short arm, however, there is a significant excess of male recombination. Twenty-seven (59%) of the markers on the map have heterozygosities greater than or equal to 0.50. The largest interval between loci on the sex-average map is 14 cM and the average marker spacing is 3 cM. Using loci on this map, one could detect linkage to a dominant disease on chromosome 16 with as few as 10-15 phase-known meioses.

• OSTI ID: 6263382
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:15; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 16
ISOTOPES
LIGHT NUCLEI
MAPPING
NUCLEI
ODD-ODD NUCLEI
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RFLPS