Cellular morphometry of the bronchi of human and dog lungs

Technical Report · Sun Sep 01 04:00:00 EDT 1991

DOI:https://doi.org/10.2172/6262282· OSTI ID:6262282

Robbins, E S

One hundred and forty-seven bronchial samples (generations 3--6) from 66 patients (62 usable; 36 female, 26 male; median age 61) have been dissected by generation from fixed surgical lung specimens obtained after the removal of pathological lesions. In addition, one hundred and fifty-six mongol dog bronchi (generations 2--6) dissected from different lobes of 26 dog lungs have also been similarly prepared. One hundred and twenty-seven human samples have been completely processed for electron microscopy and have yielded 994 electron micrographs of which 655 have been entered into the Computerized Stereological Analysis System (COSAS) and been used for the measurement of the distances of basal and mucous cell nuclei to the epithelial free surface. Similarly 328 micrographs of dog epithelium from 33 bronchial samples have been used to measure the distances of basal and mucous cell nuclei to the epithelial free surface and have been entered into COSAS. Using the COSAS planimetry program, we continue to expand our established data bases which describe the volume density and nuclear numbers per electron micrograph for 5 cell types of the human bronchial epithelial lining of men and women, as well as smokers, non-smokers and ex-smokers and similar parameters for the same 5 epithelial cell types of dog bronchi. Our micrographs of human bronchial epithelium have allowed us to analyze the recent suggestion that the DNA of lymphocytes may be subject to significant damage from Rn progeny while within the lung. Since the last progress report three papers have been submitted for publication. 17 refs., 4 tabs.

• Research Organization: New York Univ., NY (United States). Dept. of Cell Biology
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-88ER60656
• OSTI ID: 6262282
• Report Number(s): DOE/ER/60656-5; ON: DE92003421
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ALPHA DECAY RADIOISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BODY
BRONCHI
CELL CONSTITUENTS
CELL NUCLEI
COLLOIDS
DATA BASE MANAGEMENT
DAYS LIVING RADIOISOTOPES
DISPERSIONS
DISTRIBUTION
DOCUMENT TYPES
ELECTRON MICROSCOPY
EPITHELIUM
EVEN-EVEN NUCLEI
HEAVY NUCLEI
HISTOLOGY
ISOTOPES
MANAGEMENT
MICROSCOPY
MORPHOLOGY
NUCLEI
PROGRESS REPORT
RADIOISOTOPES
RADON 222
RADON ISOTOPES
RESIDUES
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SMOKES
SOLS
SPATIAL DISTRIBUTION
TISSUES
TOBACCO SMOKES