Cellular morphometry of the bronchi of human and dog lungs

Technical Report · Fri Mar 01 04:00:00 EST 1991

OSTI ID:5915854

Robbins, E S

One hundred and thirty-one bronchial samples from 62 patients have been dissected by generation from fixed surgical lung specimens obtained after the removal of pathological lesions. Complete patient records including occupational and smoking histories, as well as possible exposure to radon, are obtained. In addition, one hundred and sixty-two mongol dog bronchi dissected from different lobes of 23 dog lungs have also been similarly prepared. Ninety-four human samples have been completely processed for electron microscopy and have yielded 994 electron micrographs of which 532 have been entered into the Computerized Stereological Analysis System (COSAS) and been used for the measurement of the distances of basal and mucous cell nuclei to the epithelial free surface. Similarly 240 micrographs of dog epithelium from 31 bronchial samples have been entered into COSAS. We have, using the COSAS planimetry program, established data bases which describe the volume density and nuclear numbers per electron micrograph for 5 cell types of the human bronchial epithelial lining of men and women, as well as smokers, non-smokers and ex-smokers and similar parameters for the epithelial cell types of dog bronchi. The data are being used to develop weighting factors for dosimetry and radon risk analysis. 26 refs., 7 figs., 4 tabs.

• Research Organization: New York Univ., NY (USA). Dept. of Cell Biology
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG02-88ER60656
• OSTI ID: 5915854
• Report Number(s): DOE/ER/60656-4; ON: DE91010939
• Country of Publication: United States
• Language: English

Related Subjects

560161* -- Radionuclide Effects
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560162 -- Radionuclide Effects
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
CELL NUCLEI
COLLOIDS
DATA
DISPERSIONS
DOCUMENT TYPES
DOGS
DOSIMETRY
ELECTRON MICROSCOPY
ELEMENTS
EPITHELIUM
EXPERIMENTAL DATA
FLUIDS
GASES
INFORMATION
LUNGS
MAMMALS
MAN
MICROSCOPY
MORPHOLOGICAL CHANGES
NONMETALS
NUMERICAL DATA
ORGANS
PRIMATES
PROGRESS REPORT
RADIATION EFFECTS
RADON
RARE GASES
RESIDUES
RESPIRATORY SYSTEM
RISK ASSESSMENT
SMOKES
SOLS
TISSUES
TOBACCO SMOKES
VERTEBRATES