Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Cancer Res.; (United States)

OSTI ID:6258910

Youakim, A; Herscovics, A

The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonic adenocarcinomas. Cells were incubated with D-(2-TH)mannose or L-(5,6-TH)fucose for 24 h and treated with trypsin to release cell surface components which were then digested exhaustively with Pronase and fractionated on Bio-Gel P-6 before and after treatment with endo-beta-N-acetylglucosaminidase H. The most noticeable difference between the labeled glycopeptides from the tumor and CCL 239 cells was the presence in the former of an endo-beta-N-acetylglucosaminidase H-resistant high molecular weight glycopeptide fraction which was eluted in the void volume of Bio-Gel P-6. This fraction was obtained with both labeled mannose and fucose as precursors. However, acid hydrolysis of this fraction obtained after incubation with (2-TH)mannose revealed that as much as 60-90% of the radioactivity was recovered as fucose. Analysis of the total glycopeptides (cell surface and cell pellet) obtained after incubation with (2-TH)mannose showed that from 40-45% of the radioactivity in the tumor cells and less than 10% of the radioactivity in the CCL 239 cells was recovered as fucose. After incubation of the HCT-8R cells with D-(1,6-TH)glucosamine and L-(1- UC)fucose, strong acid hydrolysis of the labeled glycopeptide fraction excluded from Bio-Gel P-6 produced TH-labeled N-acetylglucosamine and N-acetylgalactosamine.

• Research Organization: McGill Univ., Montreal, Quebec
• OSTI ID: 6258910
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States); ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
ALDEHYDES
ANIMAL CELLS
BIOCHEMISTRY
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARCINOMAS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DIGESTIVE SYSTEM
DISEASES
GALACTOSE
GASTROINTESTINAL TRACT
GLUCOPROTEINS
HEXOSES
HYDROLYSIS
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
MANNOSE
MONOSACCHARIDES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
SACCHARIDES
SOLVOLYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS