Presence of fucosyl residues on the oligosaccharide antennae of membrane glycopeptides of human neuroblastoma cells

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Cancer Res.; (United States)

OSTI ID:5715467

Santer, U V; Glick, M C

Fucosyl residues linked alpha 1 leads to 3 or 4 to N-acetylglucosamine were found in large amounts on glycopeptides from the membranes of human tumor cells of neurectodermal origin but not on membrane glycopeptides from human fibroblasts. The fucosyl residues were detected by release of radioactive fucose from the glycopeptides with an almond alpha-L-fucosidase specific for fucosyl alpha 1 leads to 3(4)-N-acetylglucosamine. In other studies, the linkage was shown to be alpha 1 leads to 3 by nuclear magnetic resonance analysis. Glycopeptides containing these fucosyl residues from four human neuroblastoma cell lines were defined by binding to immobilized lectins. In addition, the glycopeptides from one human neuroblastoma cell line, CHP-134, were further characterized by enzyme degradation and columns calibrated for size and charge. The antennary position of fucosyl alpha 1 leads to 3-N-acetylglucosamine on the glycopeptides was demonstrated by the use of exoglycosidases and endoglycosidase D, since complete degradation to yield fucosyl-N-acetylglucosaminylasparagine was obtained only after treatment with almond alpha-L-fucosidase prior to the sequential degradation. Fucosyl alpha 1 leads to 3-N-acetylglucosamine was present on most size and charge classes of membrane glycopeptides and therefore was not limited to a few glycoproteins. Since the almond alpha-L-fucosidase cleaves fucosyl residues from glycoproteins, the physiological effects of the increased specific fucosylation on human tumors of neurectodermal origin can be examined.

• Research Organization: Joseph Stokes, Jr. Research Institute, Children's Hospital of Philadelphia, Pennsylvania
• OSTI ID: 5715467
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43:9; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
AMIDES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
ASPARAGINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DISEASES
ENZYMES
FIBROBLASTS
GLUCOPROTEINS
GLYCOSYL HYDROLASES
HEMAGGLUTININS
HEXOSAMINES
HEXOSES
HYDROGEN ISOTOPES
HYDROLASES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAGNETIC RESONANCE
MAMMALS
MAN
MEMBRANES
MONOSACCHARIDES
NEOPLASMS
NERVOUS SYSTEM
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
RESONANCE
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES