Micromolar protein concentrations and metalloprotein stoichiometries obtained by inductively coupled plasma atomic emission spectrometric determination of sulfur

Journal Article · Thu Dec 15 04:00:00 EST 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00175a008· OSTI ID:6256748

Bongers, J; Walton, C D; Richardson, D E; Bell, J U

The concentrations of several micromolar solutions of proteins with known sulfur contents were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) of sulfur in the vacuum ultraviolet. These values are compared to concentrations obtained by using spectrophotometric measurements and published specific and molar absorptivities based on various conventional methods of protein determination. The two sets of values are in close agreement, indicating that ICP-AES of sulfur is an accurate means of determining microgram quantities of proteins. Standard deviations are within 2% of the mean values obtained for data sets of six replicate measurements. Dilute buffered protein solutions may be directly pumped into the nebulizer; sample digestion and other special sample preparations are not necessary. It is also demonstrated for several metalloproteins that multielement ICP-AES is an excellent means of determining stoichiometries of bound metal ions as both protein and metal assays may be rapidly performed on a single sample.

• Research Organization: Univ. of Florida, Gainesville (USA)
• OSTI ID: 6256748
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:24; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHEMICAL ANALYSIS
DATA
DISPERSIONS
ELEMENTS
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
INFORMATION
MIXTURES
NONMETALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMA
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
SOLUTIONS
SPECTROSCOPY
STOICHIOMETRY
SULFUR
TRACE AMOUNTS