Study of factors affecting growth and cold acclimation of Vitis callus cultures
In vitro grape tissue culture initiation, growth, and cold acclimation were studied. Factors involved were genotypes, media, plant growth regulators, age, light, temperature, antioxidant, clearing and adsorbing agents, sucrose level, osmotic potential, ABA, chilling and freezing treatments. Murashige and Skoog (MS) medium containing 1 ..mu..M 2,4-d + 0.1 uM Ba, MS containing 1 uM 2,4-D, and woody plant medium containing 1 uM 2,4-D + 0.1 uM BA produced abundant callus tissue for most grape genotypes; either WPM or MS containing 1 uM BA stimulated shoot growth in all the 12 genotypes tested. Adding 1 uM abscisic acid (ABA) to the B5 medium with 1 uM 2,4-D and 0.5 uM BA enhanced growth and quality of Chancellor callus. /sup 3/H-ABA was taken up actively by callus tissue at 12 days after subculture, but by 20 d this effect disappeared. When /sup 14/C-sucrose was added to the medium. /sup 14/C level of cells reached a plateau after 48 h; this plateau was higher if ABA was also present in the medium. Cells on media containing ABA were larger in size, lighter in color, and more loosely connected.
- Research Organization:
- Minnesota Univ., Minneapolis (USA)
- OSTI ID:
- 6256648
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GRAPES
PLANT GROWTH
ABSCISIC ACID
BIOLOGICAL ADAPTATION
FREEZING
GENOTYPE
HORMONES
SACCHAROSE
TEMPERATURE EFFECTS
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AUXINS
CARBOHYDRATES
CARBOXYLIC ACIDS
DISACCHARIDES
FOOD
FRUITS
GROWTH
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
SACCHARIDES
550501* - Metabolism- Tracer Techniques
560200 - Thermal Effects